In vitro antiviral activity of stabilized chlorine dioxide containing oral care products

Abstract

Objective
To determine the in vitro antiviral activity of oral care products containing stabilized chlorine dioxide toward infectious viruses that harbor in the oral cavity. Specfically, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), SARS- CoV, human coronavirus (HCoV) 229E, influenza A (H3N2), rhinovirus type 14, adeno- virus type 5, and herpes simplex virus (HSV)
type 1 and 2 were examined.

Methods
Validated in vitro suspension virucidal assays were used. Test product was mixed with the test virus for 30, 60, or 120 s, neutralized with sodium thiosulfate, serially diluted in dilution medium in a 96-well plate and incubated in a carbon dioxide incubator for 7 days. The 50% Tissue Culture Infectious Dose per milliliter was determined.

Results
Two rinses, one oral spray and one fluoride toothpaste showed log reduction of severe acute respiratory syndrome coronavirus-2 ranging from 1.81 to 2.98 and of influenza A from 2.58 to 4.13, respectively, within 30 s of contact time; similar results were obtained at 60 s. Further, the Ultra Sensitive rinse showed 0.19, 0.75, 1.58, 1.75, 2.66, and 3.24 log reduction of severe acute respiratory syndrome coronavirus, human coronavirus 229E, rhinovirus type 14, adenovirus type 5, and herpes simplex virus type 1 and type 2, respectively, within 30 s of contact time.

Conclusion
Stabilized chlorine dioxide containing ClōSYS® oral care products reduced
the viral load of multiple viruses within 30s. The results warrant further investigation for potential in vivo applications.
View PDF Full Text